Intraperitoneal Alpha-Lipoic Acid to prevent neural damage after crush injury to the rat sciatic nerve

doi:10.1186/1749-7221-4-22

Journal of Brachial Plexus and Peripheral Nerve Injury

Volume 4

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Senoglu M
Nacitarhan V
Kurutas EB
Senoglu N
Altun I
Atli Y
Ozbag D

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Nacitarhan V
Kurutas EB
Senoglu N
Altun I
Atli Y
Ozbag D
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Research article

Intraperitoneal Alpha-Lipoic Acid to prevent neural damage after crush injury to the rat sciatic nerve

Mehmet Senoglu¹ , Vedat Nacitarhan² , Ergul Belge Kurutas³ , Nimet Senoglu⁴ , Idris Altun¹ , Yalcin Atli³ and Davut Ozbag⁵

¹Department of Neurosurgery, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey

²Department of Physical Medicine and Rehabilitation, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey

³Department of Biochemistry, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey

⁴Department of Anaesthesiology and Reanimation, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey

⁵Department of Anatomy, Kahramanmaras Sutcu Imam University Faculty of Medicine, Kahramanmaras, Turkey

author email corresponding author email

Journal of Brachial Plexus and Peripheral Nerve Injury 2009, 4:22doi:10.1186/1749-7221-4-22


Published:	25 November 2009

Abstract

Objective

Crush injury to the sciatic nerve causes oxidative stress. Alfa Lipoic acid (a-LA) is a neuroprotective metabolic antioxidant. This study was designed to investigate the antioxidant effects of pretreatment with a-LA on the crush injury of rat sciatic nerve.

Methods

Forty rats were randomized into four groups. Group I and Group II received saline (2 ml, intraperitoneally) and a-LA (100 mg/kg, 2 ml, intraperitoneally) in the groups III and IV at the 24 and 1 hour prior to the crush injury. In groups II, III and IV, the left sciatic nerve was exposed and compressed for 60 seconds with a jeweler's forceps. In Group I (n = 10), the sciatic nerve was explored but not crushed. In all groups of rats, superoxide dismutase (SOD) and catalase (CAT) activities, as well as malondialdehyde (MDA) levels were measured in samples of sciatic nerve tissue.

Results

Compared to Group I, Group II had significantly decreased tissue SOD and CAT activities and elevated MDA levels indicating crush injury (p < 0.05). In the a-LA treatment groups (groups III and IV), tissue CAT and SOD activities were significantly increased and MDA levels significantly decreased at the first hour (p < 0.05) and on the 3^rdday (p < 0.05). There was no significant difference between a-LA treatment groups (p > 0.05).

Conclusion

A-LA administered before crush injury of the sciatic nerve showed significant protective effects against crush injury by decreasing the oxidative stress. A-LA should be considered in the treatment of peripheral nerve injuries, but further studies are needed to explain the mechanism of its neuroprotective effects.